Final answer:
The first enzyme immunoassay (EIA), widely known as ELISA, was available in 1980. It is primarily performed using a blood sample from a vein, with a Western blot used as a confirmatory test.
Step-by-step explanation:
The first enzyme immunoassay (EIA), also known as ELISA (enzyme-linked immunosorbent assay), became available in 1980. The most common method for initial HIV testing includes the use of an EIA or ELISA test on a sample of blood drawn from a vein, followed by a confirmatory test, notably the Western blot, on the same blood sample. Alternate samples for ELISA might include saliva or urine. The ELISA test is often followed by a confirmatory test, such as the western blot or PCR. ELISA tests rely on antibodies to detect antigens with high specificity. When a sample contains the antigen, the test yields a color change indicating a positive result, which then often requires confirmation with more sensitive tests such as Western blot or nucleic acid amplification test (NAAT).