Final answer:
To carry out a serial dilution, start with a standard solution and create test tubes with a fixed volume. Add a fixed volume of the original culture to each test tube and mix thoroughly. Repeat this process multiple times to create a series of dilutions that bracket the desired concentration.
Step-by-step explanation:
A serial dilution is a dilution in which the concentration is divided by 10 at each interval. To carry out a serial dilution, start with a standard solution and create test tubes with a fixed volume. Add a fixed volume of the original culture to each test tube and mix thoroughly. Repeat this process multiple times to create a series of dilutions that bracket the desired concentration.
To carry out a serial dilution, you start with a standard solution of a certain concentration and create test tubes with a fixed volume, typically 9 cm^3. In each step of the dilution, a fixed volume of the original culture is added and thoroughly mixed with the dilution solution containing a certain volume.
This process is repeated multiple times, resulting in a series of dilutions that bracket the desired concentration for accurate counting.