Question

How does one carry out a serial dilution in which the concentration halves at each interval?

e.g. you start with a standard solution of 40cm^3 in volume of 1% concentration and you want to create test tubes with volume 20cm^3 each

Answer 1

To carry out a serial dilution with a halving concentration, start with a standard solution and repeatedly dilute it by half until the desired concentration is reached.

Step-by-step explanation:

To carry out a serial dilution with a halving concentration, start with a standard solution and repeatedly dilute it by half until the desired concentration is reached.

To carry out a serial dilution where the concentration halves at each interval, you can follow these steps:

1. Start with a standard solution of 40cm^3 in volume of 1% concentration.
2. Take 20cm^3 of the standard solution and add it to a new test tube containing 20cm^3 of diluent (usually a solvent like water). This is a 1:1 dilution.
3. Take 20cm^3 from the 1:1 diluted solution and add it to another 20cm^3 of diluent. This is a 1:2 dilution, resulting in half the concentration of the previous solution.
4. Repeat step 3 as many times as needed until the desired concentration is reached.