Final answer:
Feeding all rats from Litter 1 the same diet would confound genetics with diet type, making it impossible to distinguish the effects of the diet from the effects of genetic variation.
Step-by-step explanation:
If the 10 rats from Litter 1 were fed Diet A, the problem created for the experiment would be B) Genetics and type of diet would be confounded. This means that any observed differences in weight gain could not be attributed solely to the diet because the genetic makeup of the rats from the healthier first litter could play a significant role. To ensure reliable results, experimenters should randomly assign individuals from both litters to each diet, preventing any one variable from being intertwined with another and affecting the outcome of the nutritional experiment.