Question

What is the correct order of pcr stages in one cycle?

1. denaturation
2. primer annealing
3. primer extension

Answer 1

The correct order of PCR stages in one cycle is denaturation, primer annealing, and primer extension.

Step-by-step explanation:

The correct order of PCR stages in one cycle is:

1. Denaturation: Heating the DNA to break the bonds holding the two strands together, resulting in two single strands of DNA.
2. Primer Annealing: Cooling the single strands of DNA and mixing them with short DNA segments called primers, which are complementary to segments of the single DNA strands.
3. Primer Extension: The temperature is raised to the optimum temperature for the DNA polymerase enzyme, allowing for the addition of nucleotides to the primer using the single-stranded DNA as a template.

Each cycle of PCR involves these three stages and results in the doubling of DNA molecules. The thermal cycler is used to automatically cycle through the required temperatures at each stage. The denaturation, annealing, and extension stages occur at high, low, and intermediate temperatures, respectively, in order to facilitate DNA replication.