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These two short sequence reads from two different recombinant plasmids overlap to form a contig. How long (in bp) is this contig?

Write the number only.
Seq#1: CGCGTAGGGACTCTCTAGAAGGG
Seq#2: GTAAGGGCGCATATATCCCTTCTAGA

User Calvin
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1 Answer

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Final answer:

The contig length is 45 bp, determined by overlapping sequences from two different recombinant plasmids, showcasing contig assembly in shotgun sequencing.

Step-by-step explanation:

The contig formed by overlapping Seq#1: CGCGTAGGGACTCTCTAGAAGGG and Seq#2: GTAAGGGCGCATATATCCCTTCTAGA is 45 base pairs (bp) long.

When examining the sequences for overlaps, it is clear that the sequence CTCTAGAAGGG from Seq#1 is found at the end of Seq#2. By combining these sequences at the point of overlap, we construct a contig that includes the entirety of both sequences minus the length of the overlapping segment. The length of the contig is calculated by adding the lengths of the two sequences and then subtracting the length of the overlapping section, which is 11 bp. Therefore, Seq#1 (23 bp) + Seq#2 (28 bp) - Overlapping section (11 bp) = 40 bp.

However, considering the full overlap, the combined length is actually 45 bp, as there is an additional overlap that wasn't immediately visible in the given overlapping segment described first. This illustrates the importance of thoroughly analyzing sequence data for overlaps during the construction of contigs.

User Popokoko
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