Question

You are given a vial containing a white powder of agarose and are told to dissolve enough in 80 ml of 1x tae to yield a 1.2% (w/v) agarose. solution. how much agarose do you need to weigh?

Answer 1

To prepare a 1.2% (w/v) agarose solution in 80 ml of 1x TAE, you need to weigh out 0.96 grams of agarose.

Step-by-step explanation:

To prepare a 1.2% (w/v) agarose solution in 80 ml of 1x TAE, you need to determine the amount of agarose to weigh. The percentage by weight is calculated by dividing the mass of agarose by the mass of the solution and multiplying by 100%.

First, convert the desired concentration to a decimal: 1.2% = 0.012.

Then, use the formula:

Agarose (g) = (0.012)(80 ml)(1 g/ml).

Agarose (g) = 0.96 g.