Question

Identify which of the statements below is/are correct for the hydrolysis of ATP to AMP+2Pᵢ

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a) A majority of endergonic reactions that require ATP to drive them forward uses this mode of ATP hydrolysis
b) The enzyme inorganic pyrophosphatase is required to catalyze the hydrolysis of PPi to 2Pi
c) This reaction results in the hydrolysis of only the phosphoanhydride bond between alpha and beta phosphates of ATP
d) This mode of ATP hydrolysis is equivalent to the hydrolysis of 2 moles of ATP to ADP +Pi
e) The nucleophilic attack on ATP takes place at the alpha phosphorus of ATP

Answer 1

The hydrolysis of ATP to AMP and two Pi involves the cleavage of the phosphoanhydride bond between the alpha and beta phosphates of ATP. The correct answer is option c).

Step-by-step explanation:

ATP hydrolysis is a process in which ATP is converted into ADP and inorganic phosphate (Pi) through the addition of water. The hydrolysis reaction releases energy, making it exergonic. This mode of ATP hydrolysis involves the cleavage of the phosphoanhydride bond between the alpha and beta phosphates of ATP.

The correct statement for the hydrolysis of ATP to AMP and two Pi is statement (c). This reaction results in the hydrolysis of only the phosphoanhydride bond between the alpha and beta phosphates of ATP. Statement (b) is incorrect as the enzyme inorganic pyrophosphatase is not involved in the hydrolysis of PPi to 2 Pi.

Additionally, statement (d) is incorrect as this mode of ATP hydrolysis is not equivalent to the hydrolysis of 2 moles of ATP to ADP + Pi. Lastly, statement (e) is incorrect as the nucleophilic attack on ATP takes place at the beta phosphorus of ATP, not the alpha phosphorus.