Final answer:
In the mitochondria, the urea cycle enzymes carbamoyl phosphate synthetase I (CPS-I) and ornithine transcarbamylase catalyze the first two steps of ammonia conversion to urea.
Step-by-step explanation:
The two urea cycle enzymes that are located inside the mitochondria are carbamoyl phosphate synthetase I (CPS-I) and ornithine transcarbamylase (OTC). The urea cycle, also known as the ornithine cycle, is a series of biochemical reactions that convert ammonia, a highly toxic compound, into urea, which is then excreted from the body. CPS-I catalyzes the first step involving the formation of carbamoyl phosphate from ammonium ions and bicarbonate, requiring ATP and activated by N-acetyl glutamate. Subsequently, OTC catalyzes the reaction where ornithine and carbamoyl phosphate combine to form citrulline, which then moves to the cytosol for subsequent steps of the cycle.