Question

The UV-VIS spectrophotometer used by the researchers contained a detector that had low sensitivity and was unable to measure high absorbance samples. Which approach to the experiment makes the most sense with this limitation in mind?

Answer 1

When dealing with a low sensitivity detector in a UV-VIS spectrophotometer, different approaches can be taken to account for the limitation, including diluting the high absorbance samples and using a different spectroscopic technique like infrared spectroscopy. Alternatively, researchers can consider using a different analytical method like chromatography or mass spectrometry.

Step-by-step explanation:

When dealing with a low sensitivity detector in a UV-VIS spectrophotometer, there are a few approaches that can be taken to account for the limitation. One approach is to dilute the high absorbance samples to bring the absorbance within the detection range of the instrument. This can be done by preparing a series of dilutions with the solvent used in the experiment. Another approach is to use a different spectroscopic technique that can measure high absorbance samples, such as infrared spectroscopy. This technique measures the absorption of infrared radiation by the sample and can provide useful information about chemical bonds and functional groups. Lastly, if the goal is to measure the concentration of a chromophore, the researchers could consider using a different analytical method altogether, such as chromatography or mass spectrometry.