Final answer:
To verify AVOP, direct observation of test outcomes, such as changes in media or containers' appearance after autoclaving, is essential to ensure effective sterilization.
Step-by-step explanation:
To verify AVOP, which stands for Autoclave Verification and Optimization Program, three aspects are usually observed. Direct observation of the outcome of tests is one of the key elements. This involves examining the physical appearance of the materials involved, such as how the media, slide, tube, or other containers look after the process. For instance, if a growth medium in a petri dish was autoclaved, you would check for any discoloration, melting, or unexpected changes that might indicate a problem with the autoclaving process.