Final answer:
The Colonel orders Annihilation after the Na'vi start running from Home Tree.
Step-by-step explanation:
The subject of this question is English as it relates to a specific book or story. The question is asking about a character's actions in a particular situation.
In the context of the question, the character in question is likely from a book or movie where the Na'vi and Home Tree are mentioned. Given the options provided, Annihilation would be the most likely answer as it means complete destruction. This suggests that the Colonel orders the complete destruction of the Na'vi or their home.