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Which of the following enzymes will produce a blunt end (the cut site is indicated by the * in the recognition sequence)?

a. TaqI (T*CGA)
b. EagI (C*GGCCG)
c. EcoRV (GAT*ATC)
d. NsiI (ATGCA*T)

User Sebi
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1 Answer

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Final answer:

EcoRV (GAT*ATC) is the enzyme from the given options that produces a blunt end by cutting straight through the DNA sequence.

Step-by-step explanation:

The enzyme that produces a blunt end from the options given is EcoRV (GAT*ATC). This means the enzyme cuts straight through the DNA sequence at the indicated cut site, resulting in the formation of blunt ends. Restriction enzymes like EcoRV are widely used in genetic engineering and recombinant DNA technology. TaqI, EagI, and NsiI, on the other hand, all produce staggered cuts, which lead to the production of 'sticky ends' rather than blunt ends.

User Bfuoco
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