Final answer:
The dye binding method that uses an increase of absorbance at 595nm to determine the total protein concentration of a specimen is the Bradford assay (Option C).
Step-by-step explanation:
The Bradford assay is the method that uses an increase of absorbance at 595nm to determine total protein concentration.
This assay relies on the binding of Coomassie Brilliant Blue dye to proteins, which results in a shift of the dye's absorbance maximum from 465 nm to 595 nm when bound to protein.
The extent of this color change is proportional to the protein concentration, which can be measured using a spectrophotometer, and is compared against a standard curve generated from known protein concentrations to determine the protein content in the sample.
Hence, the correct answer is Option C.