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What is the basic technique used to detect unexpected antibodies with a commercial set of reagent cells?

Options:
A) Western blot
B) Polymerase chain reaction (PCR)
C) Enzyme-linked immunosorbent assay (ELISA)
D) Crossmatch test

1 Answer

2 votes

Final answer:

The basic technique used to detect unexpected antibodies in a sample using a commercial set of reagent cells is the Enzyme-linked immunosorbent assay (ELISA), which is sensitive and allows for quantification of antibodies.

Step-by-step explanation:

The basic technique used to detect unexpected antibodies with a commercial set of reagent cells is Enzyme-linked immunosorbent assay (ELISA). ELISA involves coating a plastic plate with the antigen associated with potential antibodies. A patient or test serum is then applied; if the serum contains the antibodies in question, they will bind to the antigen on the plate. After washing away non-specific bindings, a secondary antibody linked to an enzyme is added, which, in turn, binds to the first antibody if present. Upon adding a chromogenic substrate, a color change will occur, indicating the presence of the antibodies. This technique is sensitive and allows for the quantification of specific antibodies in the plasma or serum.

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