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What is the correct statement regarding the electrophoretic migration of a protein with a pI of 9.2 in an electrical field with a pH set at 10.0?

Options:
A) The protein will migrate towards the anode.
B) The protein will migrate towards the cathode.
C) The protein will remain stationary.
D) The direction of migration cannot be determined.

1 Answer

2 votes

Final answer:

The protein with a pI of 9.2 will migrate towards the cathode when subjected to an electric field at a pH of 10.0, because it will have a net negative charge.

Step-by-step explanation:

The correct statement regarding the electrophoretic migration of a protein with a pI of 9.2 in an electrical field with a pH set at 10.0 is that the protein will migrate towards the cathode. This is because the isoelectric point (pI) of a protein is the pH at which it has no net charge. When the pH of the electrical field is higher than the protein's pI, the protein will have a net negative charge and thus migrate towards the positive electrode (anode). However, in this case, since the pH is set at 10.0, the protein will have a net negative charge and will move towards the cathode, which is the negative electrode.

User Chars Davy
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