Final answer:
The item in the game "The Binding of Isaac" that grants a +1.0 Luck Up, providing a better chance to win while gambling, but does not affect all pills to become positive or neutral like in the original game, is the Lucky Toe (option B).
Step-by-step explanation:
In the game "The Binding of Isaac," the item that grants a +1.0 Luck Up, providing a better chance to win while gambling, but does not affect all pills to become positive or neutral like in the original game, is the Lucky Toe (option B).