Final answer:
In "The Binding of Isaac," The Mulligan is the item that, combined with two other fly-type items, transforms the player into the Lord of the Flies, providing flight and converting enemy flies to allies.
Step-by-step explanation:
In the popular video game "The Binding of Isaac," the item that, when picked up along with 2 other fly-type items, enables the player to transform into the Lord of the Flies is: B) The Mulligan.
Picking up The Mulligan allows Isaac to generate attacking flies when he hits enemies with his tears. If Isaac obtains two other items from the fly set, such as Forever Alone, BBF (Big Beautiful Fly), or Distant Admiration, he will then transform into the Lord of the Flies. This transformation provides Isaac with the ability to fly and the ability to convert enemy flies into blue flies which will then fight for him.