Final answer:
Activation energy is the energy required to initiate a chemical reaction. The transition state is the highest point on an energy diagram and represents the unstable intermediate state. Initial rate is the rate at which a reaction proceeds at the beginning. Steady-state kinetics studies enzyme-catalyzed reactions with a constant intermediate concentration. Km is the substrate concentration at which the reaction proceeds at half of its maximum rate. Vmax is the maximum rate of an enzyme-catalyzed reaction.
Step-by-step explanation:
Activation Energy:Activation energy is the energy required to initiate a chemical reaction. It is the minimum amount of energy required for reactant molecules to reach the transition state and convert into products.
Transition State:The transition state, also known as the activated complex, is the highest point on an energy diagram and represents the arrangement of reactant molecules in an unstable intermediate state. It is the point at which old bonds are breaking and new bonds are forming.
Initial Rate:The initial rate is the rate at which a reaction proceeds at the beginning, when the reactant concentration is highest. It is determined by measuring the change in concentration of reactants or products over a short period of time.
Steady-State Kinetics:Steady-state kinetics refers to the study of enzyme-catalyzed reactions under conditions where the concentration of an intermediate remains constant over time. It is often used to determine the kinetic parameters of enzymes, such as the rate of reaction and Michaelis-Menten constants.
Km:Km, also known as the Michaelis constant, is a measure of the substrate concentration at which an enzyme-catalyzed reaction proceeds at half of its maximum rate. It provides information about the affinity of an enzyme for its substrate.
Vmax:Vmax is the maximum rate at which an enzyme-catalyzed reaction can proceed. It is reached when all enzyme active sites are saturated with substrate.