Question

show a proper work for the following steps, TEP 1: When beginning your flowchart construction, list all possible species (from the test grid provided) to begin your flowchart. After the initial complete species list, you will have to then break things down according to Gram results. Then, after that, choose the appropriate biochemical tests to get the identification (lab exercises of weeks 10-12 will largely dictate which tests you should be looking at to narrow your choices, ideally in that order). Once you have received your Gram results, narrow down groups based on each appropriate feature or test. You only have the tests listed on the test grid to use in your identification, thus, your flowcharts! Please note that you will NOT use all the tests on the test grid for your flowchart, just the minimum to get the identification. Remember that this and only this flowchart will be used in steps 2 and 3 below—if there are errors in your flowchart, you will receive incomplete/inaccurate test results to base your final conclusions on. Ask your INSTRUCTOR, NOT anyone else, for help! To construct your flowchart, you may use the computer or do it by hand. If by computer, it must fit on one page for easier reading. Make sure everything is neat and legible. STEP 2 (Rationale) and 3 (Identification): After you have begun testing, complete the part of this sheet that tells what tests you did (in the order performed), the results you obtained, and based on that, what new list of organisms you have at each step. You will do the minimum tests to get the identification, plus 1-2 confirmation tests performed after final identification is determined. The purpose of confirmation tests is to further rule out all the other organisms you had listed at the testing step previous to the final test that got you the sample ID), AND to provide a further level of confidence that you made the right conclusion. You may do a third confirmation test if you did not need a lot of tests to get the identification—check with your instructor for help.

Answer 1

The question involves creating a flowchart using a test grid to identify bacterial species, and further refining results using Bergey's Manual and biochemical tests. API test panels can be used for rapid identification.

Step-by-step explanation:

Steps in Bacterial Identification

The process of bacterial identification begins with the construction of a flowchart outlining all possible species based on a given test grid. Initially, you categorize species based on their Gram results and then apply the appropriate biochemical tests. These tests and the order in which they are performed are dictated by lab exercises from weeks 10-12. After receiving Gram results, you refine your identification by applying more specific tests from the test grid, only using the minimum number of tests necessary to identify the species, followed by 1-2 confirmation tests.

Once you have reduced your options to a single genus, you refer to Bergey's Manual to determine the specific tests needed to differentiate and identify the species within that genus. This involves consulting the appropriate identification tables and conducting the relevant biochemical assays to obtain a metabolic profile for the species. After conducting the tests, compile the observed test outcomes to create a dichotomous key for further refinement of your results.

API test panels, which are commonly used for rapid microbial identification, can be employed during this process. These panels contain multiple biochemical tests, providing a metabolic profile used to classify bacteria. This efficient method of microbial identification is particularly useful in clinical laboratories, helping to quickly determine appropriate treatment for patients.