Final answer:
In the process of translation, a tRNA without an amino acid is released from the E (exit) site of the ribosome after it has contributed to the growing polypeptide chain.
Step-by-step explanation:
During translation, a tRNA that no longer carries an amino acid is released from the E (exit) site. This occurs after the tRNA has transferred its amino acid to the growing polypeptide chain and the ribosome has translocated. The released tRNA, now uncharged, exits the ribosome through the E site, making room for a new tRNA to enter the A (aminoacyl) site, continuing the process of protein synthesis.