Question

The annealing stage in PCR is most likely to occur at what temperature?

a) 94°C
b) 72°C
c) 55°C
d) 37°C

Answer 1

The annealing stage in PCR typically occurs at 55°C, allowing primers to bind to the target DNA sequence for successful amplification.

Step-by-step explanation:

The annealing stage in PCR is most likely to occur at what temperature? The answer is c) 55°C. During the Polymerase Chain Reaction (PCR), the annealing step is where primers attach or anneal to the complementary template strands of the DNA. This temperature is typically set at around 55°C, which allows the primers designed to flank the targeted DNA region to bind efficiently. As part of the PCR cycle, temperatures are specifically set at each step to ensure proper denaturation, annealing, and extension of DNA, with the aim of amplifying the target sequence.