Final answer:
In Shakespeare's 'Macbeth', Act 3, Scene 1, Macbeth speaks the lines in question to the murderers as he convinces them to kill Banquo.
Step-by-step explanation:
In Act 3, Scene 1 of William Shakespeare's play Macbeth, the lines "Do you find your patience so predominant in your nature that you can let this go? Your spirits shine through you" are said by Macbeth to the murderers. Macbeth is trying to persuade the murderers to kill Banquo, by suggesting that Banquo is their enemy and that their willingness to let slights pass is an indication of a patient demeanor. He also compliments their spirit, as a way to manipulate them into committing the murder.