Question

Peter adds 500 µL of bacteria to a tube containing 3 mLs of nutrient broth and then adds 100 µL of a certain disinfectant. The final concentration of the disinfectant in this tube after adding it is 5%.

Please find the following this is all I was given
C1=
C2=
V1=
V2=

Answer 1

The question is about calculating the initial concentration of a disinfectant needed to reach a 5% final concentration in a biological dilution setting

Step-by-step explanation:

The question relates to determining the final concentration of a disinfectant in a mixture after dilution with a bacterial culture and a nutrient broth in a biological setting. Using the dilution factor and C1V1=C2V2 formula, where C1 and V1 represent the initial concentration and volume of the disinfectant respectively, and C2 and V2 represent the final concentration and total volume after dilution, we can calculate the initial concentration of the disinfectant required to achieve the 5% final concentration. However, certain values needed for this calculation are missing from the student's supplied information.

In general, to achieve a uniform suspension of bacterial cells for testing, organisms are diluted or concentrated and then plated according to the desired assay. Tests such as the in-use test, MIC (Minimum Inhibitory Concentration), and MBC (Minimum Bactericidal Concentration) use these principles to assess the effectiveness of disinfectants or to estimate the number of cells in a culture.. However, not all necessary values were provided to do this. Techniques like the in-use test evaluate disinfectant effectiveness by assessing microbial growth.