Question

You want to purify a protein that has a pl of ∼9. Based on this information alone, which of the following fractionation procedures would you use?

A. Gel filtration
B. Anion Exchange
C. SDS-PAGE
D. Cation exchange
E. Affinity Chromatography

Answer 1

Anion Exchange Chromatography can be used to purify a protein with a pI of ∼9.

Step-by-step explanation:

The protein with a pI of ∼9 can be purified using Anion Exchange Chromatography. Anion exchange chromatography separates proteins based on their net charge at a specific pH. Since the protein has a pI of ∼9, it will have a net negative charge at pH∼8.6 and will interact with the positively charged resin in the column.