Final answer:
To determine the number of unique 1H NMR signals for a compound, we analyze the splitting patterns and chemical shifts detailed in the NMR data provided, indicative of different proton environments, such as the six new singlets and the three new signals replacing the H1' doublet.
Step-by-step explanation:
Estimating the number of unique 1H NMR signals for a given compound involves analyzing the molecular structure to determine if there are protons in equivalent environments. In the context of the supplied information, the presence of various characteristic chemical shifts and the formation of complexes, which result in shifts and changes in the NMR spectra, indicate different proton environments. By examining the data, such as the disappearance of the H6 singlet and the appearance of new singlets, as well as the changes in the H1' doublet, we can trace the number of unique signals expected.
For example, the disappearance of the H6 singlet and appearance of new singlets at 9.28, 9.22, 9.20, 8.93, 8.63, and 8.60 ppm signifies six distinct proton environments due to complexation. Additionally, the appearance of three new signals at 5.73, 5.39, and 5.30 ppm in place of the H1' doublet suggests three distinct chemical environments. Finally, the mention of the H8 resonances appearing as two singlets indicates two more unique environments. Without seeing the entire molecular structure, a precise count is challenging, but the shifts and splitting patterns described indicate that multiple unique 1H NMR signals would be seen for the compound being analyzed.