Final answer:
RNA primase is not involved in the resolution of Okazaki fragments in E. coli.
Step-by-step explanation:
The enzyme or enzymatic activity that is NOT involved in the resolution of Okazaki fragments in E. coli is D. RNA Primase.
Okazaki fragments are short DNA fragments that are synthesized on the lagging strand during DNA replication. These fragments are later stitched together to form a continuous strand.
In E. coli, DNA Helicase unwinds the DNA double helix, DNA Polymerase I removes the RNA primers and replaces them with DNA, and DNA Ligase seals the gaps between the Okazaki fragments.