Final answer:
RNA Polymerase is the correct answer as it is not involved in the processes at the origin of replication in E. coli; this enzyme is responsible for transcription, not DNA replication.
Step-by-step explanation:
The protein that does NOT function at the origin of replication in E. coli among the options provided is D. RNA Polymerase. The proteins and enzymes that do function at the origin of replication include A. DNA Helicase, which unwinds the DNA double helix; B. DNA Polymerase III, which is responsible for the bulk of DNA synthesis; and C. DNA
Primase, which synthesizes the RNA primers necessary for DNA polymerase to begin replication. RNA Polymerase is not involved in DNA replication but instead synthesizes RNA from a DNA template during transcription.