Final answer:
B) False. The statement suggesting that ORO dye binds more to polar molecules, implying that isopropanol was used to elute ORO for this reason, is false. In reality,
Step-by-step explanation:
The statement that isopropanol was used to perform an elution of the ORO (Oil Red O) because the ORO dye binds to the more polar molecule is false. Oil Red O is a lysochrome (fat-soluble dye) commonly used for staining of neutral triglycerides and lipids in tissues, and it has a higher affinity for less polar substances like lipids. ORO binds to less polar substances and the nonpolar nature of isopropanol helps with the elution.
The use of isopropanol, which is a less polar solvent than water, actually helps to release the hydrophobic ORO dye from tissue sections during the staining process. In other words, because ORO is a nonpolar dye, it is more soluble in less polar solvents like isopropanol, which is why isopropanol is used for the elution of ORO from stained biological samples.