Final answer:
Cholesterol is conjugated with HRP in the experiment to facilitate its detection, allowing for easy quantification using ELISA techniques.
Step-by-step explanation:
The use of cholesterol conjugated with HRP (Horseradish Peroxidase) in the experiment rather than cholesterol alone is likely due to facilitated detection. Cholesterol itself can be difficult to detect and measure directly in experimental assays. Conjugating it with HRP, an enzyme commonly used in biochemical assays, allows for easy detection through enzyme-linked immunosorbent assay (ELISA) techniques.
HRP can convert substrates into colored or fluorescent products that are easily quantifiable. This allows researchers to measure the presence and quantity of cholesterol in samples with greater ease and precision.