Question

What primary ingredients, coupled with DNA polymerase I, are needed for the in vitro synthesis of DNA?

A) DNA template, RNA primer, dNTPs
B) RNA template, DNA primer, dNTPs
C) DNA template, DNA primer, RNA nucleotides
D) DNA template, RNA primer, rNTPs

Answer 1

For in vitro DNA synthesis with DNA polymerase I, a DNA template, RNA primer, and deoxynucleotide triphosphates (dNTPs) are required.

Step-by-step explanation:

The primary ingredients needed for the in vitro synthesis of DNA, coupled with DNA polymerase I, are A) DNA template, RNA primer, dNTPs. In the DNA replication process, a DNA template provides the necessary pattern for creating the new DNA strand. An RNA primer is synthesized by primase and is essential to initiate DNA synthesis, as DNA polymerase can only add to an existing 3'-OH end. The building blocks of the new DNA strand, deoxynucleotide triphosphates (dNTPs), including ATP, GTP, TTP, and CTP, are added to the growing chain by DNA polymerase.