Final answer:
The correct option in the final answer is a. The correct order of the steps in ChIP-Seq is Cross-linking, Shearing, Immunoprecipitation, DNA Purification, Sequencing. This method is used to identify DNA sequences bound to a specific protein by fixing the protein to the DNA, fragmenting the complex, isolating specific protein-DNA complexes, purifying the DNA, and then sequencing it.
Step-by-step explanation:
The correct order of the steps in chromatin immunoprecipitation (ChIP-Seq) from first to last is a) Cross-linking, Shearing, Immunoprecipitation, DNA Purification, Sequencing. The steps are as follows:
- Cross-linking: This step involves using a chemical agent to fix proteins to DNA, which prevents them from dissociating during the procedure.
- Shearing: The DNA-protein complexes are physically broken down into smaller pieces, often by sonication or enzymatic digestion, to make the DNA of suitable length for sequencing.
- Immunoprecipitation: An antibody specific to the protein of interest is used to selectively bind and pull down the protein-DNA complexes.
- DNA Purification: The DNA is separated from the protein and other components, resulting in purified DNA fragments for sequencing.
- Sequencing: The purified DNA fragments are then sequenced to identify the DNA sequences that were bound to the protein of interest.
ChIP-Seq is a powerful technique that combines chromatin immunoprecipitation with next-generation sequencing to understand the protein-DNA interactions within the genome.
Please note that the correct option to the original question is a) Cross-linking, Shearing, Immunoprecipitation, DNA Purification, Sequencing.