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Order the steps in chromatin immunoprecipitation (ChIP-Seq) from first to last.

a) Cross-linking, Shearing, Immunoprecipitation, DNA Purification, Sequencing
b) Shearing, Cross-linking, Immunoprecipitation, DNA Purification, Sequencing
c) Immunoprecipitation, Shearing, Cross-linking, DNA Purification, Sequencing
d) Cross-linking, Immunoprecipitation, Shearing, DNA Purification, Sequencing

User Luislhl
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Final answer:

The correct option in the final answer is a. The correct order of the steps in ChIP-Seq is Cross-linking, Shearing, Immunoprecipitation, DNA Purification, Sequencing. This method is used to identify DNA sequences bound to a specific protein by fixing the protein to the DNA, fragmenting the complex, isolating specific protein-DNA complexes, purifying the DNA, and then sequencing it.

Step-by-step explanation:

The correct order of the steps in chromatin immunoprecipitation (ChIP-Seq) from first to last is a) Cross-linking, Shearing, Immunoprecipitation, DNA Purification, Sequencing. The steps are as follows:

  1. Cross-linking: This step involves using a chemical agent to fix proteins to DNA, which prevents them from dissociating during the procedure.
  2. Shearing: The DNA-protein complexes are physically broken down into smaller pieces, often by sonication or enzymatic digestion, to make the DNA of suitable length for sequencing.
  3. Immunoprecipitation: An antibody specific to the protein of interest is used to selectively bind and pull down the protein-DNA complexes.
  4. DNA Purification: The DNA is separated from the protein and other components, resulting in purified DNA fragments for sequencing.
  5. Sequencing: The purified DNA fragments are then sequenced to identify the DNA sequences that were bound to the protein of interest.

ChIP-Seq is a powerful technique that combines chromatin immunoprecipitation with next-generation sequencing to understand the protein-DNA interactions within the genome.

Please note that the correct option to the original question is a) Cross-linking, Shearing, Immunoprecipitation, DNA Purification, Sequencing.

User Amaters
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