Question

All of the following are necessary conditions of the yeast two-hybrid system except _______.

(a) a single yeast cell can harbor two different plasmids simultaneously
(b) both the binding domain and the activating domain of Gal4 retain their individual functions after being separated by recombinant DNA methods
(c) UASG must be situated between the Gal1 and Gal10 gene sequences
(d) β-galactosidase synthesis is under the control of UASG

Answer 1

The yeast two-hybrid system requires a single yeast cell to harbor two different plasmids, the binding domain and activating domain of Gal4 to retain their functions, and β-galactosidase synthesis under the control of UASG. However, the condition that is not necessary is UASG being situated between the Gal1 and Gal10 gene sequences.

Step-by-step explanation:

The necessary conditions of the yeast two-hybrid system include:

1. A single yeast cell can harbor two different plasmids simultaneously.
2. Both the binding domain and the activating domain of Gal4 retain their individual functions after being separated by recombinant DNA methods.
3. β-galactosidase synthesis is under the control of UASG.

Therefore, the condition that is not necessary is (c) UASG must be situated between the Gal1 and Gal10 gene sequences.