Final answer:
While the exact volume of blood for each bottle of culture set is not specified, typical lab practices involve transferring 2 ml of Tryptic Soy Broth into each culture tube for subculturing and using a 1:10 dilution method for bacterial culture preparation.
Step-by-step explanation:
The amount of blood to be used in each bottle of culture set is not explicitly stated in the given information; however, there are guidelines on how to conduct a dilution series using a broth such as Tryptic Soy Broth (TSB). When preparing cultures in a laboratory setting, it is essential to use aseptic technique to avoid contamination. According to the provided procedure, when performing a broth subculture, you would transfer 2 ml of broth into each sterile culture tube. In the context of diluting bacterial cultures, a 1.0 mL sample is added to 9.0 mL of sterile broth to achieve dilution factors of 1:10, 1:100, and so on. For this process, the student should follow the instructions provided by their lab manual or instructor for the required volume since the exact amount for culturing can vary based on the experimental design or the standard practices of the lab.