Final answer:
The rubber stopper of medication should not be removed as this can compromise sterility. The use of a pipette for transferring sterile liquids involves not contacting the liquid with hands, skin, or surfaces and using techniques to prevent contamination.
Step-by-step explanation:
It is generally not permissible to remove the rubber stopper from medication; such action could compromise the sterility of the medication. The only circumstances under which the stopper might be removed are those involving strict aseptic techniques, which might include using sterile gloves and tools such as a sterile clamp. However, standard practice in aseptic technique does not typically involve removing the rubber stopper entirely but instead involves disinfecting the stopper surface with alcohol before inserting a needle through the stopper to withdraw medication.
When transferring sterile liquids, such as with a pipette, tools and techniques are utilized to maintain sterility. Pre-sterilized pipettes are used to accurately transfer liquid without touching hands, skin, or lab surfaces. During the transfer process, the bottles should be held at an angle to minimize the entrance of airborne contaminants, and the opening of the tube or bottle should be briefly passed through a flame before and after the transfer to discourage contamination.