Final answer:
An unsterilized surgical assistant may touch various items and surfaces that do not penetrate sterile tissues or contact mucous membranes.
Step-by-step explanation:
An unsterilized surgical assistant may touch various items and surfaces in a surgical environment that do not penetrate sterile tissues or come into contact with mucous membranes. These include equipment such as microscopes, Bunsen burners, inoculating loops, metal forceps, pipette pumps, incubators, and refrigerators. However, it is crucial for the surgical assistant to maintain proper aseptic technique and hygiene protocols to prevent the transmission of pathogens and minimize the risk of contamination.