Final answer:
To prevent the spread of infection, gloves should be removed first after completing a venipuncture in an isolation room, followed by immediate hand hygiene and subsequent removal of other PPE according to CDC guidelines.
Step-by-step explanation:
When completing a venipuncture in an isolation room, the proper removal of personal protective equipment (PPE) is crucial to prevent the spread of infection. The Centers for Disease Control and Prevention (CDC) provides guidelines on the sequence of removing PPE. According to these guidelines, gloves should be removed first because they are the most likely to be contaminated. After carefully removing and disposing of the gloves, hand hygiene practices should be followed immediately. Subsequent steps involve the removal of face protection (goggles or face shield), gown, and then a mask or respirator if used, each step followed by proper hand hygiene to ensure maximal safety and infection control.