Question

Use the sequence from the pre-mRNA shown below to answer the following question.

5^'-GCCAUUCAGGUGAGACGUAAUGCGCUUACCUAUACAGGACCUCGGG-3^'
What would be the sequence of the spliced mRNA? (Remove the intron)

Answer 1

The spliced mRNA sequence from the pre-mRNA provided, after removing the intron marked by GU at the start and AG at the end, is 5'-GCCAUUCGUGAGACGUAAUGCGCUUACCUAUACAGGACCUCGGG-3'.

Step-by-step explanation:

The question relates to the removal of introns during the process of mRNA splicing, which is essential before protein synthesis can proceed. In the provided sequence, we need to identify the intron locations to determine the spliced mRNA sequence. Introns typically start with GU and end with AG. Searching for these markers in the sequence, we find them flanking the following segment: 5'-GCCAUUCAG...GUGAGACGUAA-3'. This represents the intron that needs to be removed.

After removing this intron, the spliced mRNA sequence would be:

5'-GCCAUUCGUGAGACGUAAUGCGCUUACCUAUACAGGACCUCGGG-3'

Notice how the sequence has been rejoined, maintaining a continuous read from 5' to 3' without the intron.