Final answer:
To find a complementary DNA sequence, match each nucleotide with its complement and reverse the sequence to run from 5' to 3'. For the example sequence 5' TGC 3', the complementary strand is 5' GCA 3'.
Step-by-step explanation:
The complementary DNA strand is synthesized in the 5' to 3' direction and will be the reverse complement of the given template sequence. For the given sequence 5' TGC 3', the complementary strand will be 5' GCA 3'. DNA polymerases add nucleotides to the 3' end of the newly forming strand, so the sequence is developed in the opposite direction to the template.
Key steps to find the complementary DNA strand:
- Identify the template DNA strand sequence.
- Match each nucleotide with its complement (A with T, T with A, G with C, C with G).
- Reverse the order of the complemented nucleotides to run from 5' to 3'.
Applying this to the example GGCTACATTCGGAA, the complementary strand will be CCGATGTAAGCCTT.