Final answer:
At least -2 standard deviations are required to separate two means for them to be considered significantly different at a 5% significance level.
Step-by-step explanation:
To determine the minimum number of standard deviations that would need to separate two means for them to be considered significantly different with a 5% significance level, we use the concept of z-scores in hypothesis testing. Commonly, a z-score of approximately 1.96 (or about 2 standard deviations) in either direction is considered the cutoff for significance at the 5% level in a two-tailed test. This corresponds to a 2.5% chance in each tail of the normal distribution. Therefore, out of the options provided, at least -2 standard deviations is the minimum required for the means to be significantly different at the 5% level.