Question

Put the following steps of the production of a recombinant vector, containing a human gene, in order

I. introduce the recombinant plasma into a bacterial cell
II. isolate the human gene and the vector DNA using restriction enzymes
III. ligate the DNA fragments to produce a recombinant plasmid
IV. grow bacterial cells on a medium containing a selective agent

A) III, I, IV, II
B) II, I, III, IV
C) III, I, II, IV
D) I, III, IV, II
E) II, III, I, IV

Answer 1

The correct order of steps to produce a recombinant vector containing a human gene is isolating the human gene and vector DNA with restriction enzymes, ligating to form a recombinant plasmid, introducing this into bacterial cells, and growing the cells on selective media.

Step-by-step explanation:

To produce a recombinant vector containing a human gene, the correct order of steps is:

1. Isolate the human gene and the vector DNA using restriction enzymes.
2. Ligate the DNA fragments to produce a recombinant plasmid.
3. Introduce the recombinant plasmid into a bacterial cell.
4. Grow bacterial cells on a medium containing a selective agent.

Therefore, the correct sequence is E) II, III, I, IV. This process begins with the isolation of the human gene of interest and vector DNA, followed by cutting with restriction enzymes that produce compatible ends. This is followed by ligation using DNA ligase to form the recombinant plasmid which is then introduced into bacterial cells, a process known as transformation. Finally, transformed bacteria are grown on selective media to ensure that only cells with the recombinant DNA survive and can be further studied.