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Which of the following restriction enzyme sites would produce sticky-end fragments (the arrow represents the cutting site)

A) CCC/GGG
B) GA/ATT
C) GG/ATC
D) AA/GCT
E) C/CGG

User Ashtom
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1 Answer

4 votes

Final answer:

Option B (GA/ATTC) is the restriction enzyme site that will produce sticky-end fragments due to its staggered cutting pattern creating single-stranded DNA overhangs.

Step-by-step explanation:

The restriction enzyme sites that would produce sticky-end fragments are those where the enzyme cuts in a way that leaves overhanging single-stranded DNA. These overhangs can base pair with complementary sequences on other DNA molecules with sticky ends, allowing for the potential ligation and formation of recombinant DNA. Among the provided options, the one that would produce sticky ends is B) GA/ATTC, since the cut is staggered and results in DNA fragments with overhangs that are complementary to each other. These ends can anneal with other fragments cut by the same restriction enzyme.

The other options would result in blunt end cuts or are not palindromic recognition sites with overhangs.

User Noemi
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