Question

All of the following are required for PCR except

A) fluorescent amino acid
B) double stranded DNA molecules or cDNA
C) DNA polymerase
D) deoxyribonucleotides
E) primers

Answer 1

A fluorescent amino acid is not required for PCR. PCR needs double-stranded DNA or cDNA (B), DNA polymerase (C), deoxyribonucleotides (D), and primers (E) but not fluorescent amino acids, which are more relevant to protein labeling. The answer is A) fluorescent amino acid.

Step-by-step explanation:

Polymerase Chain Reaction (PCR) is a powerful molecular biology technique used to amplify DNA. Among the options given, the odd one out is fluorescent amino acid.

PCR primarily requires double-stranded DNA or cDNA as the template (B), DNA polymerase (C) to catalyze the synthesis of the new DNA strand, deoxyribonucleotides (D) as the building blocks, and primers (E) that serve as starting points for DNA synthesis.

Fluorescent amino acids are not directly related to PCR; they are more commonly associated with techniques like fluorescence microscopy or protein labeling. So, option A is not a necessary component for the PCR process.