Question

"Synthesis of cDNA requires the use of ______

A) fluorescent synthetic nucleotides.
B) reverse transcriptase.
C) DNA ligase.
D) restriction enzymes.
E) agarose."

Answer 1

The synthesis of cDNA requires the enzyme B. reverse transcriptase, which transcribes RNA into a complementary DNA strand.

Step-by-step explanation:

The synthesis of cDNA requires the use of reverse transcriptase. This enzyme is essential for converting RNA into DNA by using RNA as a template to synthesize a complementary DNA strand. This process is employed by retroviruses, like HIV, which bring reverse transcriptase into a host cell to replicate their RNA into cDNA, and is also a crucial tool in recombinant DNA technology for cDNA cloning and other genetic engineering applications. Neither fluorescent synthetic nucleotides, DNA ligase, restriction enzymes, nor agarose are directly involved in the synthesis of cDNA from an RNA template.

The synthesis of cDNA requires the use of reverse transcriptase. Reverse transcriptase is an enzyme that uses RNA as a template to produce a DNA copy. It recognizes both DNA and RNA templates and has DNA polymerizing activity, allowing it to synthesize the cDNA strand.