Question

Which of the following procedures might be used to detect the presence of genetic sequences of a virus in a sample? ______

A) Southern blotting
B) genome mapping
C) Southern blotting or PCR
D) creation of a gene library
E) PCR

Answer 1

To detect the presence of genetic sequences of a virus in a sample, Southern blotting and PCR are used. Southern blotting is suited for detecting DNA sequences, while PCR amplifies DNA, facilitating detection. Southern blotting is ideal for detecting DNA polymorphism related to sickle cell anemia.The right answer is C)

Step-by-step explanation:

The procedures that might be used to detect the presence of genetic sequences of a virus in a sample are Southern blotting and PCR (Polymerase Chain Reaction). Southern blotting involves separating DNA fragments on a gel, transferring them to a nylon membrane, and incubating them with a DNA probe that is complementary to the sequence of interest. On the other hand, PCR is a technique that can amplify tiny amounts of DNA, making it easier to detect the presence of a virus.

In the context of sickle cell anemia, which affects hemoglobin in red blood cells, the most useful technique for detecting polymorphism in a sample would be Southern blotting. This technique looks specifically at DNA, where the mutation causing sickle cell anemia would be present.