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A laboratory protocol lists the following ingredients: 1 g sucrose, 16.4 g Na2HPO4, 1.5 g (NH4)3PO4, 0.02 g CaCO3, KNO3, water to 1 liter and autoclave. This recipe is for a ________ medium. ______

A) reducing agar
B) complex broth
C) defined agar
D) complex enrichment broth
E) defined broth

1 Answer

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Final answer:

The recipe is for a complex medium because it includes components whose exact chemical compositions are not known. The absence of agar in the list suggests it is for a liquid medium, making 'complex broth' the likely answer.

Step-by-step explanation:

The laboratory protocol lists ingredients for a medium that includes various compounds and water that will be autoclaved. Among the ingredients are sucrose and various salts, but the presence or absence of agar, which is needed for solid media, isn't mentioned. The medium is complex because it contains extracts and digests of substances like meat or yeast, whose exact chemical compositions are not known, as opposed to a defined medium where all chemical components are known and precisely measured. Without info about agar being included, we cannot specify whether it's meant for a broth or agar medium; however, the absence of a mention of agar suggests it could be for a liquid medium. Thus, complex broth seems to be the correct answer.

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