Final answer:
The property of a protein least likely to be affected by pH changes is the primary structure.
Step-by-step explanation:
The property of a protein that is least likely to be affected by changes in pH is the primary structure. The primary structure refers to the specific sequence of amino acids in a protein. This sequence is determined by the order of nucleotides in the gene that codes for the protein and is not significantly influenced by changes in pH.vConversely, a hydrolysis reaction is the process where a peptide bond is broken down. This happens with the addition of a water molecule, which effectively splits the amino acids apart. These two types of reactions are essential in the synthesis and degradation of proteins.