Final answer:
As cells are used from the deeper part of the epidermis towards the surface, b) they lose their nuclei.
Step-by-step explanation:
As cells are used from the deeper part of the epidermis towards the surface, they lose their nuclei. The outermost layer of the epidermis, called the stratum corneum, is made up of dead keratinocytes that have lost their nuclei, organelles, and most of their cytoplasm. These cells become filled with the protein keratin and form a protective, waterproof layer. So, the correct answer is B) They lose their nuclei.