Final answer:
To prepare a smear from a broth culture, take a sample of the liquid broth and spread it onto a glass slide. To prepare a smear from a culture on solid media, take a sample of the colony and spread it onto a glass slide.
Step-by-step explanation:
When preparing a smear from a broth culture, you would first take a sample of the liquid broth using a sterile inoculating loop. Then, you would spread the sample onto a glass slide and allow it to air dry. Finally, you would heat fix the slide to affix the bacterial cells to the slide.
When preparing a smear from a culture on solid media, such as an agar plate, you would take a sample of the colony using a sterile inoculating loop. Then, you would spread the sample onto a glass slide and allow it to air dry. Finally, you would heat fix the slide to affix the bacterial cells to the slide.