Question

In addition to the two-hybrid assay, what is another type of assay that could be used to determine interactions between proteins?

Option 1: Co-immunoprecipitation
Option 2: Pull-down assay
Option 3: Western blot
Option 4: All of the above

Answer 1

Co-immunoprecipitation(1) is another valuable technique used to determine protein-protein interactions, in addition to the two-hybrid assay.

Step-by-step explanation:

Another type of assay that could be used to determine interactions between proteins is co-immunoprecipitation(1).

Co-immunoprecipitation (Co-IP) is a technique that allows the identification of proteins that interact with a target protein in a cellular context. In Co-IP, the target protein is immunoprecipitated using an antibody, along with any interacting proteins. The co-immunoprecipitated proteins can then be identified through analysis, such as mass spectrometry, or by using specific antibodies against known interacting proteins.

So, in addition to the two-hybrid assay, Co-immunoprecipitation is another valuable technique to determine protein-protein interactions.